CF™532 is an orange colored fluorescent dye with absorption and emission maxima at 527 and 558 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF™532 is not only bright and photostable but also highly water-soluble. As a result, CF™532 antibody conjugates retain excellent solubility and yield the brightest fluorescence among antibody conjugates prepared from spectrally similar dyes. For example, CF™532 labeled goat anti-mouse IgG is significantly brighter than the same antibody labeled with Alexa Fluor® 532 at a similar degree of labeling (Figures 2, 3).